Publications at Michigan State University

  • Orlando MA, Pouillon HJT, Mandal S, Kroos L, and Orlando BJ. Substrate engagement by the intramembrane metalloprotease SpoIVFB. Nat Commun 15, 8276 (2024). https://doi.org/10.1038/s41467-024-52634-6

  • Mardikoraem M, Eaves JN, Belecciu T, Pascual N, Aljets A, Orlando BJ, Shapiro EM, and Woldring DR. Predicting Inhibitors of OATP1B1 via Heterogeneous OATP-Ligand Interaction Graph Neural Network (HOLI-GNN). (2024). bioRxiv 2024.10.01.615464;

  • George NL, Bennett EC, Orlando BJ. Guarding the walls: the multifaceted roles of Bce modules in cell envelope stress sensing and antimicrobial resistance. J Bacteriol. (2024) PMID: 38869304.

  • Orlando BJ. Perception and Protection: The Role of Bce-modules in Antimicrobial Peptide Resistance. Biochim Biophys Acta Biomembr. (2024). PMID: 38460782

  • George NL, Orlando BJ. Architecture of a complete Bce-type antimicrobial peptide resistance module. Nat Commun. (2023). PMID: 37393310; PMCID: PMC10314905.

  • George NL, Schilmiller AL, Orlando BJ. Conformational snapshots of the bacitracin sensing and resistance transporter BceAB. Proc Natl Acad Sci U S A. (2022). PMID: 35349335.

  • Orlando BJ, Dominik PK, Roy S, Obgu C, Erramilli SK, Kossiakoff AA, and Vecchio AJ. Development, Structure, and Mechanism of Synthetic Antibodies that Target Claudin and Clostridium perfringens Enterotoxin Complexes. J Biol Chem. (2022) PMID: 35952760

Publications at Harvard Medical School

  • Fan M, Zhang J, Tsai CW, Orlando BJ, Rodriguez M, Xu Y, Liao M, Tsai MF, Feng L. (2020) Structure and mechanism of the mitochondrial Ca2+uniporter holocomplex. Nature. 582(7810):129-133. PMID: 32494073

  • Orlando BJ, and Liao M. (2020) ABCG2 transports anticancer drugs via a closed-to-open switch. Nature Commun. 2020;11(1):2264. PMID: 32385283

  • Thélot F, Orlando BJ, Li Y, and Liao M. (2020) High-resolution views of lipopolysaccharide translocation driven by ABC transporters MsbA and LptB2FGC. Curr Opin Struct Biol. Apr 23;63:26-33. PMID: 32335504

  • Chew TA*, Orlando BJ*, Zhang J*, Latorraca NR, Wang A, Hollingsworth SA, Chen D, Dror RO, Liao M, and Feng L. (2019) Structure and mechanism of the cation-chloride co-transporter NKCC1. Nature. 572, 488–492. PMID: 26506510. * equal contribution

  • Giessen TW, Orlando BJ, Verdegaal AA, Chambers MG, Gardener J, Bell DC, Birrane G, Liao M, and Silver PA. (2019) Large protein organelles form a new iron sequestration system with high storage capacity. eLife 8:e46070. PMID: 31282860

  • Li Y*, Orlando BJ*, and Liao M. (2019) Structural basis of lipopolysaccharide extraction by the LptB2FGC complex. Nature. 567, 486–490. PMID: 30894744. *equal contribution

  • Fan C*, Fan M*, Orlando BJ*, Fastman NM*, Zhang J, Xu Y, Chambers MG, Xu X, Perry K, Liao M, and Feng L. (2018) X-ray and cryo-EM structures of the mitochondrial calcium uniporter. Nature 559, 575-579. PMID: 29995856. *equal contribution

    Previous Publications…

  • Dong L*, Yuan C*, Orlando BJ*, Malkowski MG, and Smith WL (2016) Fatty Acid Binding to the Allosteric Subunit of Cyclooxygenase-2 Relieves a Tonic Inhibition of the Catalytic Subunit. Journal of Biological Chemistry 291, 25641-25655. PMID: 27756840. *equal contribution

  • Orlando BJ and Malkowski MG. (2016) Crystal structure of rofecoxib bound to human cyclooxygenase-2. Acta Crystallogr F Struct Biol Commun. 72(Pt 10): p. 772-776. PMID: 27710942

  • Orlando BJ and Malkowski MG. (2016) Substrate Selective Inhibition of COX-2 by Fenamic Acid Derivatives is Dependent on Peroxide Tone. Journal of Biological Chemistry. 291(29):15069-81. PMID: 27226593

  • Lucido MJ, Orlando BJ, Vecchio AJ, and Malkowski MG. (2016) The Crystal Structure of Aspirin Acetylated Human Cyclooxygenase-2: Insight into the Formation of Products with Altered Stereochemistry. Biochemistry. 55(8):1226-38. PMID: 26859324

  • Orlando BJ, Georgieva ER, Borbat PP, Freed JH, and Malkowski MG. (2015) Pulsed Dipolar Spectroscopy Reveals that Tyrosyl Radicals are Generated in Both Monomers of the COX-2 Dimer. Biochemistry. 54, 7309-7312. PMID: 26636181

  • Orlando BJ, Lucido MJ, and Malkowski MG. (2015) The structure of ibuprofen bound to cyclooxygenase-2. Journal of Structural Biology, 189, 62-66. PMID: 25463020

  • Orlando BJ, McDougle DR, Lucido MJ, Eng ET, Graham LA, Schneider C, Stokes DL, Das A, and Malkowski MG. (2014) Cyclooxygenase-2 Catalysis and Inhibition in Lipid Bilayer Nanodiscs. Arch. of Biochemistry and Biophysics, 546, 33-40. PMID: 24503478

  • Vecchio AJ, Orlando BJ, Nandagiri R, and Malkowski MG. (2012). Investigating Substrate Promiscuity in Cyclooxygenase-2: The role of Arg-120 and Residues Lining the Hydrophobic Groove. Journal of Biological Chemistry. 287:24619-24630. PMID: 22637474